Tuesday, February 4, 2020

Post candidacy: all the crap in lab

It is annoying to argue anything with my PI.


Regardless of what I argue, how I argue, what data I present, he is always glued to his stubborn opinion.

Last week, he forced a change in imaging medium on the ground that the CO2 supply was changed thus the imaging medium needs to be changed accordingly. Despite all the arguments sounds logical, the fact remains that this might be incompatible with my experiments due to my specific procedure that took place prior to the imaging.

Today I was able to demonstrate this in live-cell imaging.

Yet he came back with all these arguments that these two cells are different and I shall not change back to my old reagent, even though the old reagent consistently makes my cells respond to stimulus and have a stable phenotype.

I could never convince him that the cells in this cell line are heterogeneous, which they are, and they are known to be, and I have seen enough at my hand.

I don't know what to do when he gave all this shit.

He always use "PIs are expected to be suspicious and challenge their trainee" as an excuse, while being an asshole around, trashing my work, while doing nothing to help.

His instinct is not to think, but fall on his "common sense", which only God knows where are based on and presumably has something to do with Jackson County, MI, and then start to argue against you, instead of support your experiments.

One of the postdoc once said jerks breed like rabbits. I think they are more likely to breed like mice, and definitely imbreed in academia, so I guess nothing is new.

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